Determining absence or presence of
Manual
AID
AIH
Post Vasectomy
Sperm Analysis
Semen
Specimen must arrive within 24 hours of collection. Send specimen Monday through Thursday only and not the day before a holiday. If holiday falls on a Saturday, holiday will be observed on the preceding Friday. Sunday holidays are observed on the following Monday. Specimen should be collected and packaged as close to shipping time as possible. Laboratory does not perform testing on weekends.
Include the following information with the specimen:
-Semen volume
-Number of days of sexual abstinence
Patient Preparation: Patient should have 2 to 7 days of sexual abstinence at the time of semen collection for accurate results.
Container/Tube: Semen Analysis Kit (T178)
Specimen Volume: Total ejaculate
See Specimen Required
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Semen | Ambient |
Determining absence or presence of
Following a vasectomy, sperm may be found in the semen for 6 weeks to 3 months or longer. Regular ejaculation (every 3-4 days) may eliminate sperm from the reproductive tract more quickly.
To check for the absence of sperm, semen should be evaluated for the presence of sperm 3 months postvasectomy and after a minimum of 20 ejaculations. Because the sperm count may be very low, the semen is centrifuged for concentration purposes.
A negative result from 1 well-mixed postvasectomy semen specimen generally indicates that the use of contraception is no longer necessary. Occasional cases have been reported where postvasectomy semen analysis shows intermittent presence of rare nonmotile sperm in the semen.(1)
Zero sperm seen
Patients may stop using other methods of contraception when examination of 1 well-mixed postvasectomy semen specimen shows azoospermia or rare nonmotile sperm (RNMS) (< or = 100,000 nonmotile sperm/mL).
The risk of pregnancy after vasectomy is approximately 1 in 2000 for men who have postvasectomy azoospermia or postvasectomy semen analysis (PVSA) showing RNMS.(1)
If >100,000 nonmotile sperm/mL persist beyond 6 months after vasectomy, then trends of serial PVSAs and clinical judgment should be used to decide whether the vasectomy is a failure and whether repeat vasectomy should be considered.(1)
Vasectomy should be considered a failure if any motile sperm are seen on PVSA at 6 months after vasectomy, in which case repeat vasectomy should be considered.
No significant cautionary statements
1. Sharlip ID, Belker AM, Honig S et al; American Urological Association: Vasectomy: AUA guideline. J Urol. 2012 Dec;188(6 Suppl):2482-2491. doi: 10.1016/j.juro.2012.09.080
2. WHO laboratory manual for the examination and processing of human semen. 5th ed. WHO Press; 2010
3. WHO laboratory manual for the examination and processing of human semen. 6th ed. World Health Organization; 2021
Seminal fluid is examined microscopically for sperm. Specimen is centrifuged if no sperm are seen and re-examined for the presence or absence of sperm. If sperm are present, the number and motility are assessed and reported.(Keel BA, Webster BW: CRC Handbook of the Laboratory Diagnosis and Treatment of Infertility. Boca Raton, FL, CRC Press, 1990)
Monday through Friday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
89321
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
POSV | Post Vasectomy Check, Semen | 50677-4 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
ABSTN | Abstinence | 10587-4 |
CLST1 | Collection Site | 56816-2 |
VL53 | Semen Volume | 3160-9 |
CENT | Centrifuged | 8100-0 |
SPML1 | Sperm/mL | 51623-7 |
MOTIL | Motility | 6800-7 |
GR3 | Grade | 29990-9 |
CMMT4 | Comment | 48767-8 |