Test Id : EHOLG
Entamoeba histolytica Antibody, Serum
Useful For
Suggests clinical disorders or settings where the test may be helpful
As an adjunct in the diagnosis of extraintestinal, invasive amebiasis
Method Name
A short description of the method used to perform the test
Enzyme-Linked Immunosorbent Assay (ELISA)
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
Amebiasis
Amebic Antibody
Amebic Serology
Amoeba Histolytica
Amoeba, Serum
Amoebiasis Antibody
E Histolytica Antibody, Serum
Entamoeba Histolytica Antibodies
Entamoeba Histolytica Serology
Parasite Serologies-done by clinic
EHOLG
Specimen Type
Describes the specimen type validated for testing
Serum
Ordering Guidance
Direct detection of Entamoeba histolytica in fecal specimens is recommended to diagnose intestinal amebiasis. See OPE / Ova and Parasite, Travel History or Immunocompromised, Feces or OAPNS / Ova and Parasite, Microscopy, Varies.
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Forms
If not ordering electronically, complete, print, and send an Infectious Disease Serology Test Request (T916) with the specimen.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
0.4 mL
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat-inactivated specimen | Reject |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 30 days |
Useful For
Suggests clinical disorders or settings where the test may be helpful
As an adjunct in the diagnosis of extraintestinal, invasive amebiasis
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Amebiasis is an infection caused by the protozoan parasite, Entamoeba histolytica. The infection is acquired by ingestion of cysts in fecally contaminated food or water; excystation and infection occur in the large intestine. After excystation, trophozoites attach to the intestinal wall and excrete extracellular enzymes that enable invasion of the mucosa and spread to other organs, especially the liver and lung where abscesses may develop.
Amebiasis (or amebic dysentery) can cause bloody diarrhea accompanied by fever and prostration. White and red blood cells are found in the stool. Liver abscess can develop several weeks to months later producing hepatomegaly and fever.
Serology may be particularly useful in supporting the diagnosis of invasive disease with E histolytica, which is most commonly associated with amebic liver abscess. Serology should not be used to identify or diagnose amebic dysentery due to poor sensitivity in acute, noninvasive disease.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Negative
Reference values apply to all ages.
Interpretation
Provides information to assist in interpretation of the test results
Negative: No antibodies to Entamoeba histolytica detected. This assay is intended for assessment of invasive amebiasis. Repeat testing in 2 to 3 weeks if clinically indicated.
Equivocal: Recommend follow-up testing in 10 to 14 days if clinically indicated.
Positive: Results are suggestive of current or past infection with Entamoeba histolytica. Direct detection of E histolytica in stool or other specimen sources is recommended to diagnose acute amebiasis.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Previous episodes of intestinal amebiasis may produce a positive serology.
Serologic results should be used as an aid in diagnosis and should not be interpreted as diagnostic by themselves.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1. Bruckner DA. Amebiasis. Clin Microbiol Rev. 1992;5(4):356-369. doi: 10.1128/CMR.5.4.356
2. Petri WA, Haque R, Moonah SN: Entamoeba species, including amebic colitis and liver abscess. In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020:3273-3286
Method Description
Describes how the test is performed and provides a method-specific reference
Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, a horseradish peroxidase-labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step, unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulfuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450/620 nm is read using an enzyme-linked immunosorbent assay microwell plate reader.(Package insert: Entamoeba histolytica ELISA IgG Test kit. Gold Standard Diagnostics; GSD-ENTG-120705.F; 01/12/2021)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Tuesday, Thursday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
86753
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| EHOLG | E. histolytica Ab, IgG, S | 22285-1 |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| EHOLG | E. histolytica Ab, IgG, S | 22285-1 |