Test Catalog

Test Id : CULAF

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Culture for Genetic Testing, Amniotic Fluid

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Overview

Test Catalog

Useful For
Suggests clinical disorders or settings where the test may be helpful

Producing amniocyte cultures that can be used for genetic analysis

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing amniocytes for prenatal genetic testing.

 

Once confluent flasks are established, the amniocyte cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.

Method Name
A short description of the method used to perform the test

Cell Culture

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Amniotic Fluid Culture/Genetic Test

Aliases
Lists additional common names for a test, as an aid in searching

Amniotic Fluid Culture for Genetic Testing

Cell culture, Amniotic fluid

Culture for Genetic Testing, Amniotic Fluid

Genetic Testing Culture, Amniotic Fluid

Saving cells for possible genetic testing

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing amniocytes for prenatal genetic testing.

 

Once confluent flasks are established, the amniocyte cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.

Specimen Type
Describes the specimen type validated for testing

Amniotic Fld

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

Provide a reason for testing with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

 

The additional tests desired must be indicated on the request form that accompanies the specimen.

ORDER QUESTIONS AND ANSWERS

Question ID Description Answers
CG767 Reason for Referral

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Optimal timing for specimen collection is during 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation are also accepted.

 

Container/Tube: Amniotic fluid container

Specimen Volume: 5 to 10 mL

Collection Instructions:

1. Discard the first 2 mL of amniotic fluid. If the culture will be performed in conjunction with other cytogenetic testing, such as CHRAF / Chromosome Analysis, Amniotic Fluid or CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling, a total of 25 to 30 mL will be needed.

4. Unavoidably, about 1% to 2% of mailed-in specimens are not viable.

5. Bloody specimens are undesirable.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.

5 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Amniotic Fld Refrigerated (preferred)
Ambient

Useful For
Suggests clinical disorders or settings where the test may be helpful

Producing amniocyte cultures that can be used for genetic analysis

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing amniocytes for prenatal genetic testing.

 

Once confluent flasks are established, the amniocyte cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Fetal cells obtained by amniocentesis (amniocytes) are used for a wide range of laboratory tests. Prior to testing, the cells may need to be cultured to obtain adequate numbers of amniocytes.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Not applicable

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Interfering factors:

-Inadequate amount of specimen may not permit adequate analysis

-Exposure of the specimen to temperature extremes (freezing or >30 degrees C) may destroy cells and interfere with attempts to culture cells

-Improper packaging may result in broken, leaky, and contaminated specimens during transport

-Transport time should not exceed 2 days

-Contamination by maternal cells may interfere with attempts to culture cells and may cause interpretive problems

Clinical Reference
Recommendations for in-depth reading of a clinical nature

Arsham MS, Barch MJ, Lawce HJ, eds. The AGT Cytogenetics Laboratory Manual. 4th ed. Wiley-Blackwell; 2017

Method Description
Describes how the test is performed and provides a method-specific reference

The specimen is split into 8 to 10 separate culture dishes. The cells are grown on coverslips in culture media for 4 to 10 days. The cultures are trypsinized into 2 to 3 T25 tissue culture flasks or 1 to 2 T75 tissue culture flasks.(Arsham MS, Barch MJ, Lawce HJ, eds. The AGT Cytogenetics Laboratory Manual. 4th ed. Wiley-Blackwell; 2017:175-178)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

23 to 24 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Cell cultures: 6 months; Any remaining supernatant or whole fluid aliquots: 14 days after results reported.

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their account representative. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

Not Applicable

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

88235

88240

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
CULAF Amniotic Fluid Culture/Genetic Test 103593-0
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
52304 Result Summary 50397-9
52306 Interpretation 69965-2
52305 Result 82939-0
CG767 Reason for Referral 42349-1
52307 Specimen 31208-2
52308 Source 31208-2
52309 Method 85069-3
54641 Additional Information 48767-8
52310 Released By 18771-6

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports